Journal: BMC Cancer
Article Title: Oncolytic adenovirus type 11-induced ferroptosis of esophageal squamous cell carcinoma cells involves in mitochondrial impairment and the mTOR pathway
doi: 10.1186/s12885-026-15735-7
Figure Lengend Snippet: The PI3K/Akt/mTOR pathway is essential for HAdV-11 replication in ESCC cell lines. ( A ) Cells were treated with HAdV-11 for 72 h at an MOI of 2 PFU/cell, the mRNA expression levels were determined using quantitative polymerase chain reaction (qPCR). ( B , D , E , F , H and I ) Cells were treated with HAdV-11 for 72 h at an MOI of 2 PFU/cell, and Western blot analysis of mTOR, p-DRP1, PARK2, LC3 and GPX4 expression. ( C ) Cells were pretreated with Temsirolimus (CCI-779) (10 nM) for 2 h, and then infected with HAdV-11 at an MOI of 2 for 48 h. The mitochondrial mass was estimated by MTG staining. ( H ) After pretreatment with 0.5 µM mTOR agonist (MHY1485) for 2 h, cells were infected with HAdV-11 at a MOI of 2 for 48 h. Subsequently, the cells were stained with either C11-BODIPY or Fe 2+ and quantified using flow cytometry. ( J ) Cells were pretreated with CCI-779 for 2 h, and then infected with HAdV-11 at an MOI of 2 for 72 h. Infectious virus production was assessed by titration on JH293 cells and the titer as PFU/cell calculated. The half maximal effective concentration (EC50) for each sample was calculated. Data are cumulative results from 3 experiments ( A , J ) or are representative of 3 independent experiments ( B , C , D , E , F , G , H , I ). Data are presented as mean ± SD ( A , C , E , F , G , H , I , J ). Differences analyzed using a one-way ANOVA with Bonferroni’s post hoc correction ( C , E , F , G , H , I ) or two-tailed t-test ( A , J ) ( n = 3)
Article Snippet: Mitochondrial division inhibitor 1 (Mdivi-1), Ferrostatin-1, Erastin, MK2206, LY294002 and Temsirolimus (CCI-779) were purchased from MedChem Express (Shanghai, China).
Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Infection, Staining, Flow Cytometry, Virus, Titration, Concentration Assay, Two Tailed Test
